Fabrication and Optimization of a 25μm Glucose Sensor

Microelectrodes have been used for over 30 years of glucose biosensor history. Two different types of glucose biosensors were developed using 176μm and 25μm diameter Pt wire cylindrical microelectrodes. The 176 μm Pt wire type microelectrodes were modified with non-conductive polymers and glucose oxidase enzyme. The lifetime of biosensors was over 50 days and had negligible response to interference species such as ascorbate and urate. The 25μm Pt wire microelectrode was coated with glucose oxidase and conductive polymer, polypyrrole by chemical polymerization. Nafion was used to eliminate interferences. The 25 μm polypyrrole based glucose sensor had the ability to measure in vivo glucose.